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M-SNAKES is a robust, reliable and freely available software for spot finding and signal extraction from microarray images. Spot detection uses the model of a virtual, contractive and elastic closed chain. This method is based upon the active contour model (snakes; Koss et al. 1987, Int. J. Comput. Vision, 1, 321-331) and was recently used to detect cell edges in Dictyostelium discoideum (Dormann et al. 2002, Cell Motil. Cytoskeleton, 52, 221-230). The present approach has been implemented in Microsoft Visual Basic 6.0 (SP4).
Institut für Biochemie Köln, Zentrum Biochemie
University of Cologne
Figure 1: M-SNAKES Interface.
Download of M-SNAKES (Zip file, 333 KB). The zip-file consists of additional OCX-files that have to be installed in the system-folder of your computer if they are not present (windows\system32).
M-SNAKES deals with 8 bit greyscale image data in BMP or JPEG format obtained by scanning of the microarray. To generate a stable, robust and user friendly software most of the parameters are fixed. Parameters that have to be adjusted individually are the image-force, the dimensions of the microarray and its sub-arrays and the mean distances between the spots.
September 12, 2013
Institute of Biochemistry I, Cologne
Suggestions and wishes: Gudrun Konertz
Voice: +49 221 4786930