of frequency distributions of
slices of different
exist to determine a tissue
in respect of the real distribution of the cell composition by the
nuclear size. Problems also exist within those group s of scientists in
respect of the frequency distribution of the size of nuclear sections.
An other problem was and is it, to determine mathematically the section
frequency distribution of a number of sphere, different in size. From
literature, it is well known as the so called 'tomatoes salad problem'*.
A relative simple
computer program is able to
produce a frequency distribution of any sphere mixtures: It has to cut
statistically the spheres into slices of defined thickness (e.g. 1) and
to collect and distribute the slices in a histogram: e.g. G1-nuclei
(vol. = 1000, t = 48 hr.) 2400, G2-nuclei (vol. =2000, t = 3 hr.) 150,
S-nuclei (vol. linearly increasing from 1000 to 2000, t = 6 hr.) 300.
Measuring the nuclear sections of a certain tissue (diameter or area)
delivers data which, processed in a histogram can be interpreted by
comparison with a corresponding simulation.
* Lit: G.
Bach 1959, 1964, 1965; Elias
1954; Glaser 1968; for reference see: Anton, H.J. und Voit E.
Microscopica Acta 84 1981, 17-23 (publ. list. 79)